BBa_K415502

BBa_K415502 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K415502
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura Deming, Joy Jiao, Adrian Slusarczyk
Date created: 2010-10-26 11:00:00
Date modified: 2015-05-08 01:12:27

SMAD5 L1L2 MammoBlock Entry Vector



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K415502_sequence (Version 1)

    Description

    SMAD5 is a osteoblast differentiation protein shown to act downstream of BMP2 to promoter osteoblast differentiation.

    Notes

    We PCR'ed insert DNA to add attB sites to either side, then used a BP recombination to create the L1L2 Entry Vector

    Source

    Human SMAD5 cDNA was obtained from OpenBiosystems.

    Sequence Annotation Location Component / Role(s)
    Start
    Stop
    RUNX2 cDNA
    		18,20
    1413,1415
    18,1415
    		start_codon feature/start
    feature/stop stop_codon
    CDS feature/protein

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K415502/1
     

    Attachments

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