ACCsynth

BBa_K417001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K417001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ben Porebski, Andrew Perry

Date created: 2010-10-19 11:00:00

Date modified: 2015-05-08 01:12:28

1-aminocyclopropane-1-carboxylate (ACC) synthase

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K417001_sequence (Version 1)

Description

This is the protein coding sequence of 1-aminocyclopropane-1-carboxylate synthase, which catalyses the formation of 1-aminocyclopropane-1-carboxylate and methylthioadenosine from S-adenosyl methionine (AdoMet).

(S-adenosyl-L-methionine <=> 1-aminocyclopropane-1-carboxylate + methylthioadenosine).

This enzyme is commonly referred to as ACC synthase.

Notes

Removal of the C-terminal amino acids. According to White, M. F., Vasquez, J., Yang, S. F., & Kirsch, J. F. (1994). PNAS, 91(26), 12428-32. (http://www.ncbi.nlm.nih.gov/pubmed/7809054) - this truncation has been reported to have no effect on specific activity.

Source

Malus domestica (Apple), codon optimized for E. coli, with the C-terminal amino acids -YYNVPEVNGGSQSSHLSHSRRQSLTKWVSRLSFDDRGPIPGR* removed

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start	1,3	feature/start, start_codon
ACC Synth	1,1290	feature/cds, CDS
Stop	1288,1290	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K417001/1

Member of these Collections

• //cds
• //cds/enzyme
• //function/biosynthesis
• iGEM Parts Registry