ACCsynth

BBa_K417001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K417001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ben Porebski, Andrew Perry
Date created: 2010-10-19 11:00:00
Date modified: 2015-05-08 01:12:28

1-aminocyclopropane-1-carboxylate (ACC) synthase



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K417001_sequence (Version 1)

Description

This is the protein coding sequence of 1-aminocyclopropane-1-carboxylate synthase, which catalyses the formation of 1-aminocyclopropane-1-carboxylate and methylthioadenosine from S-adenosyl methionine (AdoMet).

(S-adenosyl-L-methionine <=> 1-aminocyclopropane-1-carboxylate + methylthioadenosine).

This enzyme is commonly referred to as ACC synthase.

Notes

Removal of the C-terminal amino acids. According to White, M. F., Vasquez, J., Yang, S. F., & Kirsch, J. F. (1994). PNAS, 91(26), 12428-32. (http://www.ncbi.nlm.nih.gov/pubmed/7809054) - this truncation has been reported to have no effect on specific activity.

Source

Malus domestica (Apple), codon optimized for E. coli, with the C-terminal amino acids -YYNVPEVNGGSQSSHLSHSRRQSLTKWVSRLSFDDRGPIPGR* removed

Sequence Annotation Location Component / Role(s)
Start
ACC Synth
Stop
1,3
1,1290
1288,1290
feature/start start_codon
feature/cds CDS
feature/stop stop_codon
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K417001/1