Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Katharina Zwicky
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:12:28
CheB (AarI C-part)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K422004_sequence (Version 1) |
Description
The chemotactic network consists of membrane (methyl accepting chemotaxis proteins: MCPs) and intracellular proteins (Che) as signal transducers and receptors. MCPs sense a minimal difference in input concentration and transduce this information unit to the repsective proteins CheW and CheA, which located inside the cell. The autophosphorylation of CheA mediated by the MCPs is the key step in tumbling induction in response to increased repellent or decreased attractant concentration. The methylation state of the MCPs is influenced by the methyltransferase CheR (transfers a methylgroup to a protein) and the demethylase CheB(cleaves a methyl group off a protein). CheA phosphorylates CheY which then diffuses through the cytoplasm to the flagellar motor protein FliM which as a response induces tumbling. The phosphatase CheZ regulates the signal termination via dephosphorylation of CheYp (the p stands for the phosphorylated form of CheY).Notes
BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. CheR-A is an A-part compatible for N-Terminal fusion. For more information see http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning.Source
Escherichia coli genome| Sequence Annotation | Location | Component / Role(s) |
| AarI N-terminal overhang AarI C-terminal overhang CheB C-part | 12,15 1062,1065 16,1061 | feature/misc sequence_feature feature/misc sequence_feature feature/cds CDS |