| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K426013_sequence (Version 1)
|
Description
A similar part exists in the Registry of standard biological parts, but this part sequence differs by 1 mutation and 3 extra AA at N-term. This part encodes a DNA modifying enzyme. This part is associated with Zinc Finger Nuclease devices. A zinc finger nuclease is an engineered protein derived from two zinc finger DNA binding proteins and cutting domains usually from a type IIS restriciton enzyme. The special thing about them is that 1) they bind long sequences of DNA, long enough that it might be unique in a eukaryotic genome, and 2) zinc finger proteins that bind to most sequences can be generated somewhat easily. So, you can use a zinc finger nuclease to introduce a ds break at a specific user-defined site in a eukaryotic genome if the protein is delivered to the nucleus of the cell. Eukaryotic ds breaks stimulate homologous recombination. So, if you deliver both the ZFN and a DNA homologous to the target site, you get insertion of your DNA into that site of the genome.
Notes
NA
Source
Synthetic
