Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Joseph Sexton Jr.
Date created: 2010-08-23 11:00:00
Date modified: 2015-05-08 01:12:29
Histidinol Dehydrogenase
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K431006_sequence (Version 1) |
Description
Histidinol dehydrogenase is an enzyme needed for the biosynthesis of the amino acid histidine. It specifically catalyzes the reaction L-histidinol + H2O+ 2 NAD+ L-histidine + 2 NADH. Because histidine is necessary for the survival of the cell, a system for selectivity can be designed by engineering an auxotrophic mutant strain with a deficient copy of the his4 gene. Synthetic biology operations are then possible by constructing a plasmid providing both the functional his4 and the part of interest. In histine absent media, cells will only grow following successful transformationNotes
In order to fit all standards, site directed mutagenesis must be preformed at 2 Xba1 sites and 1 BamH1 siteSource
NCBICrane, D.I; Gould S.J. (1994) ???The Pichia pastoris HIS4 gene: the Pichia pastoris HIS4 gene: nucleotide sequence, creation of a non-reverting his4 deletion mutant, and development of HIS4-based replicating and integrating plasmids??? Current Genetics Vol 26(5-6) pg443-450
| Sequence Annotation | Location | Component / Role(s) |
| Start Codon Histidinol Dehydrogenase Stop Sequence | 1,3 4,2514 2515,2520 | start_codon feature/start feature/protein CDS feature/stop stop_codon |