| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_K431007_sequence (Version 1)
|
Description
Pichia pastoris initially gained attention as an expression system after recognition of its methylotrophic capabilities and the high levels of alcohol oxidase(AOX) present when induced by methanol. By isolating the promoter (pAOX1 )responsible for AOX production, this system can be utilized to obtain high yields of heterologous protein. The promoter provides not only high expression but very tight regulation as well. Vectors providing protein expression under the control of pAOX1 are the primary system used for P. pastoris
Notes
This part was obtained by designing primers and isolating it from the organisms DNA. There were no unwanted restriction enzyme sites to eliminate
Source
Isolated from Pichia pastoris
