Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Caballero
Date created: 2011-10-20 11:00:00
Date modified: 2015-05-08 01:12:30
pUC18R6KT-miniTn7BB-Gm-bistable_switch
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K510044_sequence (Version 1) |
Description
This plasmid allows the integration of a transcriptional flip-flop into bacterial chromosomes using the miniTn7BB characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.For more information about the function of the miniTn7 BioBrick toolkit, visit the page of the pUC18R6KT-miniTn7BB-Gm plasmid (BBa_K510012).
Notes
The BBa_K177038 BioBrick was inserted within the BCS of pUC18R6KT-miniTn7BB-Gm by EcoRI and PstI clonning.Source
The pUC18R6KT vector was amplified by PCR using as template a pTNS2 plasmid (GenBank accession number: AY884833) provided by Herbert P. Schweizer. The primers were designed to flanked the pUC18R6KT with SfiI restriction sites and allow the insertion of the mini-Tn7-Gm. Primers: attcGGCCTAGGCGGCCgtcgttttacaacgtcgtgac and attcGGCCGCCTAGGCCggaagcataaagtgtaaagcctg. The miniTn7BB-Gm minitransposon was synthesized commercially, and then digested at the flanking SfiI sites and cloned into SfiI-digested pUC18R6KT PCR products.| Access | Instance | Definition |
| public public | BBa_K510012 BBa_K177038 | BBa_K510012 BBa_K177038 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K510012 BBa_K177038 | 1,4549 4558,8490 | BBa_K510012 BBa_K177038 |