BBa_K511000

BBa_K511000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K511000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grant Robinson

Date created: 2011-09-24 11:00:00

Date modified: 2015-05-08 01:12:31

Hef1a-LacOid Promoter MammoBlock

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K511000_sequence (Version 1)

Description

This part encodes a promoter with low-level, constitutive activity that can be repressed by variants of the LacI transcriptional repressor.

As shown in Figures 1 and 2 below, cotransfection of a construct containing a yellow fluorescent protein (EYFP) driven by the Hef1a-LacO promoter with a construct containing a LacI-KRAB transcriptional repressor driven by the TRE-Tight promoter proves that it is possible to repress Hef1a-LacO expression with the LacI-KRAB LacI variant, which includes a Kruppel-associated box motif previously shown to silence chromatin and thereby inhibit transcription.

Notes

When sequencing constructs that include this part, it is important to realize that the Hef1a-LacOid promoter is roughly a kilobase long; therefore, promoter-gene pairs will require either internal sequencing primers or sequencing from the gene side of the construct to be useful.

Source

This part is a synthetic promoter formed from the human elongation factor 1a promoter sequence and a LacOid operator that has been computationally shown to bind to the LacI repressor protein more tightly than the wildtype LacO operator.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Hef1-alpha Promoter	1,1275	promoter, feature/promoter
Lac O1	201,221	feature/operator, operator
Lac Oid	272,291	feature/operator, operator

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K511000/1

Member of these Collections

• iGEM Parts Registry