BBa_K511002

BBa_K511002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K511002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grant Robinson

Date created: 2011-09-24 11:00:00

Date modified: 2015-05-08 01:12:31

minCMV-4xMnt Promoter MammoBlock

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K511002_sequence (Version 1)

Description

This part encodes a promoter that is slightly inducible by the Mnt-VP16 transactivator and off otherwise.

As shown in Figures 1 and 2 below, induction with the CI434-VP16 transactivator results in a subtle increase in fluorescence from a construct containing a red fluorescent protein (mKate) driven by the minCMV-4xMnt promoter.

Notes

Not applicable.

Source

This promoter is a synthetic construct composed of the minimal sequence required for transcription from the immediate-early cytomegalovirus (CMV) promoter preceded by four binding sites for the Mnt protein adapted from bacteriophage P22.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Mnt Box	1,67	feature/operator, operator
Mnt Box	72,138	operator, feature/operator
Mnt Box	143,209	operator, feature/operator
Mnt Box	214,280	operator, feature/operator
minCMV Promoter	290,347	promoter, feature/promoter

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K511002/1

Member of these Collections

• iGEM Parts Registry