BBa_K511203

BBa_K511203 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K511203

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grant Robinson

Date created: 2011-09-26 11:00:00

Date modified: 2015-05-08 01:12:31

rtTA3 Tetracycline-Inducible Transactivator MammoBlock

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K511203_sequence (Version 1)

Description

This part encodes a transactivator composed of the DNA-binding domain for the tetracycline operator sequence and the viral VP16 transactivation domain; this protein has been engineered over previous versions to be more sensitive to tetracycline and its analogues.

Figures 1 and 2 below show expression from a promoter with six tetracycline response elements (TRE-Tight) in the presence of rtTA3 and various levels of doxycycline, a tetracycline analogue.

Notes

Not applicable.

Source

This protein is a heavily engineered fusion between the tetracycline operator binding domain from TetR and the viral VP16 transactivation domain from the herpes simplex virus.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
rtTA3	1,708	feature/protein, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K511203/1

Member of these Collections

• iGEM Parts Registry