Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:31
LacI-KRAB Repressor MammoBlock
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K511205_sequence (Version 1) |
Description
This part encodes a repressor protein that binds tightly to the lactose operator originating from Escherichia coli. This protein is a fusion between the LacI repressor and the Kruppel-associated box (KRAB), a domain previously shown to strongly repress transcription from affected promoters via chromatin silencing. This repressor has been codon-optimized for mammalian expression and is capable of exerting very strong repression on promoters with internal lactose operator sites. Because LacI-KRAB-mediated repression can last for days due to its chromatin remodeling activity, LacI-KRAB is recommended for situations in which a change in transcriptional activity must be stable for extended periods of time, such as a permanent switch.Notes
Not applicable.Source
This protein is a fusion between a mammalian codon-optimized version of the LacI repressor originally derived from Escherichia coli and the Kruppel-associated box derived from a human zinc finger repressor.| Sequence Annotation | Location | Component / Role(s) |
| LacI KRAB Domain | 1,1080 1081,1464 | feature/protein CDS feature/tag tag |