Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:31
Notch-Gal4-ESN Juxtacrine Signaling Receptor MammoBlock
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_K511300_sequence (Version 1) |
Description
This part encodes a major mammalian cell-signaling protein, the Notch-1 receptor, engineered to replace the original 12xCSL-binding transactvation domain with a minimal version of the Gal4 transactivator (Gal4-ESN). In the presence of the Delta ligand protein from another cell, this transactivation domain is cleaved and released to the Notch producing cell's nucleus. In contrast, if Delta is present on the Notch producing cell, the Delta protein will cis-inhibit the Notch receptor, desensitizing the receptor to activation from external Delta molecules. This receptor is singularly useful for cell patterning, lineage decision-making, and other forms of signaling-intensive processes.Notes
Because of the substantial size of the Notch-Gal4-ESN part (roughly 6000 base pairs), it is recommended that internal primers are designed for sequencing of constructs including this part. Additionally, we have noted some difficulty in producing Notch-Gal4 through polymerase chain reaction (PCR) methods. We recommend using 5% DMSO to produce this part via PCR.Source
This part is derived from the human Notch-1 receptor and the Gal4 transactivation protein from Saccharomyces cerevisiae.| Sequence Annotation | Location | Component / Role(s) |
| Notch-1 Extracellular Domain Transmembrane Linker Gal4-ESN Minimal Transactivation Domain | 1,5223 5224,5289 5290,6072 | feature/protein CDS conserved_region feature/conserved CDS feature/protein |