Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:31
Notch-Gal4-VP16 Juxtacrine Signaling Receptor MammoBlock
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_K511301_sequence (Version 1) |
Description
This part encodes a major mammalian cell-signaling protein, the Notch-1 receptor, engineered to replace the original 12xCSL-binding transactvation domain with the Gal4-VP16 transactivator (Gal4-ESN). In the presence of the Delta ligand protein from another cell, this transactivation domain is cleaved and released to the Notch producing cell's nucleus. In contrast, if Delta is present on the Notch producing cell, the Delta protein will cis-inhibit the Notch receptor, desensitizing the receptor to activation from external Delta molecules. This receptor is singularly useful for cell patterning, lineage decision-making, and other forms of signaling-intensive processes.This version of the Notch signaling protein is expected to induce much stronger levels of activation than those seen in the figures below or those documented in recent works by Spriznak, et al.
Notes
Not applicable.Source
This part is derived from the human Notch-1 receptor, the DNA-binding domain of the Gal4 transactivation protein from Saccharomyces cerevisiae, and the viral VP16 transactivation domain from the herpes simplex virus.| Sequence Annotation | Location | Component / Role(s) |
| Notch-1 Extracellular Domain Transmembrane Linker Gal4-VP16 Transactivation Domain | 1,5223 5224,5289 5290,5970 | feature/protein CDS feature/tag tag CDS feature/protein |