Types | DnaRegion
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Roles | engineered_region
Signalling
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Sequences | BBa_K511302_sequence (Version 1)
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Description
This part encodes a fusion between the Delta-3 ligand and the monomeric mCherry red fluorescent protein.
Notes
In the original plasmid provided, the Delta-mCherry sequence did not have a stop codon, resulting in plasmid read-through of about nine additional amino acids; our Delta-mCherry includes a stop codon immediately after the mCherry sequence. Both plasmids are phenotypically correct.
Source
This part is composed of the Delta-3 ligand from Rattus norvegicus and the synthetic mCherry fluorescent protein engineered from the green fluorescent protein isolated from Aequorea victoria. Additionally, the sequence for this part was included in a plasmid provided to the MIT iGEM 2011 team courtesy of the Elowitz lab at Caltech.