BBa_K511302

BBa_K511302 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K511302
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:31

Delta-mCherry Juxtacrine Signaling Ligand MammoBlock



Types
DnaRegion

Roles
engineered_region

Signalling

Sequences BBa_K511302_sequence (Version 1)

Description

This part encodes a fusion between the Delta-3 ligand and the monomeric mCherry red fluorescent protein.

Notes

In the original plasmid provided, the Delta-mCherry sequence did not have a stop codon, resulting in plasmid read-through of about nine additional amino acids; our Delta-mCherry includes a stop codon immediately after the mCherry sequence. Both plasmids are phenotypically correct.

Source

This part is composed of the Delta-3 ligand from Rattus norvegicus and the synthetic mCherry fluorescent protein engineered from the green fluorescent protein isolated from Aequorea victoria. Additionally, the sequence for this part was included in a plasmid provided to the MIT iGEM 2011 team courtesy of the Elowitz lab at Caltech.

Sequence Annotation Location Component / Role(s)
Delta-3 Juxtacrine Signaling Ligand
mCherry Fluorescent Protein
Stop Codon
1,2142
2143,2850
2851,2853
CDS feature/protein
CDS feature/protein
feature/stop stop_codon
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BBa_K511302/1