Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:31
AVPR2-TEVs-Gal4-VP16 Signaling Receptor MammoBlock
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_K511400_sequence (Version 1) |
Description
This part encodes a fusion protein consisting of the AVPR2 receptor, the recognition sequence for the tobacco etch virus (TEV) protease, and the Gal4-VP16 transactivator.Notes
The TEV recognition sequence encoded in the rough center of this part is flanked on both sides by sequences that code for three glycines. This feature was designed to serve as a homologous region allowing for facile substitution of either a receptor or a transactivator around the TEV recognition sequence using the Gibson ligation assembly method.Source
This part includes human arginine vasopressin receptor 2 (AVPR2), the tobacco etch virus (TEV) protease recognition sequence, the DNA-binding domain from the Gal4 transactivator of Saccharomyces cerevisiae, and the VP16 transactivation domain from the herpes simplex virus.| Sequence Annotation | Location | Component / Role(s) |
| AVPR2 Receptor Flexible TEV Recognition Sequence Gal4-VP16 Transactivator | 1,1113 1114,1152 1153,1830 | feature/protein CDS feature/tag tag feature/protein CDS |