BBa_K511601

BBa_K511601 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K511601

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jonathan Chien

Date created: 2011-10-07 11:00:00

Date modified: 2015-05-08 01:12:31

N-Cadherin-eGFP Cell Adhesion MammoBlock

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K511601_sequence (Version 1)

Description

This part encodes a fusion protein. The first part, N-Cadherin is kmown to be instrumental in intercellular adhesion and tissue integrity. Like all cadherins, N-Cadherin mediates adhesion with five extracellular, calcium-binding repeats and an intercellular domain that makes contacts with the actin cytoskeleton of mammalian cells. The second part is an enhanced green fluorescent protein (eGFP), allowing for expression of both cell adhesion and an identifying marker with a single part.

Notes

Not applicable.

Source

The N-Cadherin sequence of this part was isolated from a murine source, and the eGFP fluorescent reporter is an engineered version of the original green fluorescent protein from Aequorea victoria.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
N-cadherin	1,2721	feature/protein, CDS
eGFP	2722,3443	feature/protein, CDS
stop codon	3441,3443	stop_codon, feature/stop

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K511601/1

Member of these Collections

• iGEM Parts Registry