| Types | DnaRegion
|
| Roles | Plasmid_Backbone
plasmid_vector
|
| Sequences | BBa_K512003_sequence (Version 1)
|
Description
pCDF-1b carries a T7 promoter and lac operator to control transcription, a replication origin derived from CloDF13, and streptomycin/spectinomycin antibiotic resistance (SmR). It also encodes an N-termi- nal His???Tag?? sequence followed by an enterokinase (Ek) cleavage site and an optional C-terminal S???TagTM sequence. Unique sites are shown on the circle map. pCDF-1b is compatible with pET vectors (ColE1 origin), pRSF vectors (RSF1030 replication origin), and pACYC derived plasmids (P15A replica- tion origin) carrying compatible antibiotic resistance markers. Sequencing can be performed using the T7 Promoter Primer (Cat. No. 69348-3) and AS S???Tag 18mer Primer (Cat. No. 71262-3) or T7 Terminator Primer (Cat. No. 69337-3). (Taken from Novagen)
Notes
This is the plasmid backbone to which BBa_K512002 is inserted to by NcoI and Acc65I.
Source
This is an engineered vector.
