fLuc

BBa_K518000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K518000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Masato Ohgishi

Date created: 2011-09-14 11:00:00

Date modified: 2015-05-08 01:12:33

RBS + firefly luciferase + d.terminator

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K518000_sequence (Version 1)

Description

Luciferase from firyfly (Photinus pyralis). Firefly luciferase emits light by the oxidation of D-luciferin, its substrate. Luciferase can be used as a measuring tool when combined with other cis-elements (regions regulating the expression of genes on the same DNA molecule; promoters and other protein-binding domains), because of its highly quantitative performance.

Notes

RBS (medium) B0032, firefly luciferase CDS I712019, double-terminator B0014

Source

From BioBrick parts.

Components

Access	Instance	Definition
public	BBa_B0032	BBa_B0032
public	BBa_I712019	BBa_I712019
public	BBa_B0014	BBa_B0014

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0032	1,13	BBa_B0032
BBa_I712019	20,1672	BBa_I712019
BBa_B0014	1681,1775	BBa_B0014

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K518000/1

Member of these Collections

• //classic/reporter
• //function/reporter/light
• iGEM Parts Registry