Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mun Ching Lee, Sylvia Ispasanie
Date created: 2011-07-28 11:00:00
Date modified: 2015-05-08 01:12:33
malS for BioSandwich
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K523003_sequence (Version 1) |
Description
This is a version ofThere are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. This part is intended to be used with Edinburgh 2011's "BioSandwich" protocol, in which a BglII site before the coding sequence, and a SpeI site after the coding sequence (i.e. the one in the suffix) are used for assembly.
Notes
The part was made using the strategy outlined inThe part was created from a lab E. coli strain using primers:
- ACT AGATCT atg aaa ctc gcc gcc tgt ttt c
- Forward; adds BglII site; starts at start codon.
*ACT ACTAGT ACC ctg ttg ccc tgc cca gac g
- Reverse; adds SpeI site; RFC23 and BioSandwich compliant; no stop codons, adds a glycine.