BBa_K523009

BBa_K523009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K523009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sylvia Ispasanie, Mun Ching Lee

Date created: 2011-08-03 11:00:00

Date modified: 2015-05-08 01:12:33

Exoglucanase for BioSandwich

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K523009_sequence (Version 1)

Description

This is a modified version of BBa_K118022 for use with the BioSandwich protocol of Edinburgh 2011.

BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.

Notes

N/A.

Source

From BBa_K118022.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
TC	1334,1335	sequence_feature, feature/misc
BglII	1,4	sequence_feature, feature/misc
Exoglucanase from aa 43	5,1333	CDS, feature/cds
ATG	5,5	feature/start, start_codon
GAC to GGC	153,153	feature/mutation, sequence_alteration
s_mutation	256,256	point_mutation, feature/s_mutation

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K523009/1

Member of these Collections

• iGEM Parts Registry