BBa_K523010

BBa_K523010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K523010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mun Ching Lee, Sylvia Ispasanie, Allan Crossman

Date created: 2011-08-03 11:00:00

Date modified: 2015-05-08 01:12:33

β
-glucosidase for BioSandwich

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K523010_sequence (Version 1)

Description

This is a modified version of BBa_K392008 for use with the BioSandwich protocol of Edinburgh 2011.

BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.

Notes

N/A.

Source

From BBa_K392008.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BglII	1,4	sequence_feature, feature/misc
ATG	5,5	start_codon, feature/start
B-glucosidase	5,1456	CDS, feature/cds
GG	1457,1458	feature/misc, sequence_feature
CGC to TGC	137,137	feature/mutation, sequence_alteration

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K523010/1

Member of these Collections

• iGEM Parts Registry