BBa_K523011

BBa_K523011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K523011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sylvia Ispasanie, Mun Ching Lee

Date created: 2011-08-03 11:00:00

Date modified: 2015-05-08 01:12:33

Endoglucanase for BioSandwich

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K523011_sequence (Version 1)

Description

This is a modified version of BBa_K118023 for use with the BioSandwich protocol of Edinburgh 2011.

BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.

Notes

For compatibility with BioSandwich, an internal BglII site had to be removed by site-specific mutagenesis.

Source

From BBa_K118023

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BglII	1,4	feature/misc, sequence_feature
GG	1262,1263	feature/misc, sequence_feature
Endoglucanase from aa 32	5,1261	CDS, feature/cds
CAG to CAA	1006,1006	feature/s_mutation, point_mutation
ATG	5,5	feature/start, start_codon
GCT to GCA	10,10	feature/s_mutation, point_mutation
CCC to CCA	13,13	point_mutation, feature/s_mutation
GGC to GGA	16,16	point_mutation, feature/s_mutation

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K523011/1

Member of these Collections

• iGEM Parts Registry