BBa_K523013

BBa_K523013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K523013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sylvia Ispasanie, Mun Ching Lee
Date created: 2011-09-04 11:00:00
Date modified: 2015-05-08 01:12:33

Plac + INP-EYFP



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K523013_sequence (Version 1)

    Description

    Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP) under the control of the Lac promoter.

    Notes

    Made using the BioSandwich protocol of Edinburgh 2011.

    Source

    Assembled from parts BBa_K523000, BBa_K523008, and BBa_K523012.

    Sequence Annotation Location Component / Role(s)
    Spacer
    Spacer
    Plac - LacZ
    rbs
    ATG
    INP
    Spacer (Linker)
    EYFP
    Spacer
    INP-EYFP fusion
    TAATAA
    -10
    -35
    LacZ
    rbs
    		1,32
    597,625
    38,589
    611,614
    623,623
    632,1555
    1565,1600
    1607,2329
    2337,2364
    623,2323
    2324,2324
    309,314
    286,291
    359,589
    348,351
    		feature/misc sequence_feature
    feature/misc sequence_feature
    sequence_feature feature/dna
    feature/rbs ribosome_entry_site
    feature/start start_codon
    sequence_feature feature/dna
    feature/misc sequence_feature
    sequence_feature feature/dna
    sequence_feature feature/misc
    feature/cds CDS
    stop_codon feature/stop
    feature/promoter promoter
    promoter feature/promoter
    CDS feature/cds
    feature/rbs ribosome_entry_site

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K523013/1
     

    Attachments

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