BBa_K524202

BBa_K524202 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K524202
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: HO Yuan Heng Trevor
Date created: 2012-08-18 11:00:00
Date modified: 2015-05-08 01:12:34

IPTG inducible Cre recombinase



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_K524202_sequence (Version 1)

    Description

    Generator of Cre recombinase. A leaky inducible promoter R0010 and strong RBS B0034 were employed to drive strong expression of Cre recombinase upon addition of IPTG to medium.

    Notes

    The lacI repressor was not included in the design, since the construct was expected to function in strains with function lacI, e.g. DH10b.

    Source

    Constructed by assembling BioBrick parts J04500 and J61047.

    Access Instance Definition
    public
    public
    		BBa_J04500
    BBa_J61047
    		BBa_J04500
    Cre

    Sequence Annotation Location Component / Role(s)
    BBa_J04500
    BBa_J61047
    		1,220
    227,1263
    		BBa_J04500
    Cre

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K524202/1
     

    Attachments

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