BBa_K530001

BBa_K530001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K530001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Wolozny

Date created: 2011-07-31 11:00:00

Date modified: 2015-05-08 01:12:35

CRTE

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K530001_sequence (Version 1)

Description

Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.

Notes

Part was PCR amplified from the genomic DNA created from the experiment and paper: High-Level Production of Beta-Carotene in Saccharomyces Cerevisiae by Successive Transformation with Carotenogenic Genes from Xanthophyllomyces Dendrorhous (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1932764/).

Source

Found in the genomic sequence of Xanthophyllomyces Dendrorhous. http://www.ncbi.nlm.nih.gov/nuccore/AY177204.1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start Codon	1,3	start_codon, feature/start
Stop Codon	1129,1131	feature/stop, stop_codon
ORF	1,1131	feature/protein, CDS
Forward Primer	1,25	feature/primer_binding, primer_binding_site
Reverse Primer	1105,1131	feature/primer_binding, primer_binding_site

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K530001/1

Member of these Collections

• iGEM 2018 Distribution Plate 6
• iGEM 2018 Distribution Plate 6 Well 11J
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM 2019 Distribution Plate 4
• iGEM 2019 Distribution Plate 4 Well 11J
• iGEM 2019 Part Library
• iGEM 2019 Plasmid pSB1C3
• iGEM Parts Registry