Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Wolozny
Date created: 2011-07-31 11:00:00
Date modified: 2015-05-08 01:12:35
CRTE
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K530001_sequence (Version 1) |
Description
Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.Notes
Part was PCR amplified from the genomic DNA created from the experiment and paper: High-Level Production of Beta-Carotene in Saccharomyces Cerevisiae by Successive Transformation with Carotenogenic Genes from Xanthophyllomyces Dendrorhous (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1932764/).Source
Found in the genomic sequence of Xanthophyllomyces Dendrorhous. http://www.ncbi.nlm.nih.gov/nuccore/AY177204.1| Sequence Annotation | Location | Component / Role(s) |
| Start Codon Stop Codon ORF Forward Primer Reverse Primer | 1,3 1129,1131 1,1131 1,25 1105,1131 | start_codon feature/start feature/stop stop_codon feature/protein CDS feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site |