Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alexander Aaring
Date created: 2011-09-22 11:00:00
Date modified: 2015-05-08 01:12:36
Constitutive promoter and RBS BBa_K081005 + Caulobacter optimized dspB + rsaA C
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K531010_sequence (Version 1) |
Description
Constitutive promoter and RBS from the Registry BBa_K081005, suspected N-acetylglucosaminidase dspB codon optimized for Caulobacter, and the C-terminal secretion tag from Caulobacter's S-layer associated protein, RsaA.Notes
Stop codon at the end of dspB was removed to ensure expression of the rsaA C-terminal secretion tag.Source
BBa_K081005 was designed by Lorenzo Pasotti and Paolo Magni from Team UNIPV-Pavia 2008. IDT synthesized dspB so as to codon optimize it for expression in Caulobacter crescentus. The C-terminal secretion tag from rsaA is from the genome of the Caulobacter lab strain CB15N.| Access | Instance | Definition |
| public public public | BBa_K081005 BBa_K531004 BBa_K531001 | BBa_K081005 BBa_K531004 BBa_K531001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K081005 BBa_K531004 BBa_K531001 | 1,58 67,1151 1160,1519 | BBa_K081005 BBa_K531004 BBa_K531001 |