BBa_K533002

BBa_K533002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K533002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ZHANG Yunxiao
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:12:36

OmpA-HIV protease site-SH3



    • Details

    Types
    DnaRegion

    Roles
    Device

    engineered_region

    Sequences BBa_K533002_sequence (Version 1)

    Description

    Added HIV protease site between OmpA and SH3 for easier release.

    Notes

    The HIV protease site is embedded in the protein so that a proteolytic cleavage can release the binding cassette.

    Source

    OmpA is from BL21(DE3) E. Coli genomic DNA.
    SH3 is from human Grb2 protein.
    HIV protease site is from a former part.

    Sequence Annotation Location Component / Role(s)
    T7 promoter
    BBa_I712015
    lacI
    OmpA
    SH3
    		1,18
    634,675
    20,44
    87,633
    676,857
    		feature/promoter promoter
    engineered_region feature/BioBrick
    feature/operator operator
    CDS feature/cds
    CDS feature/cds

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K533002/1
     

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