Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ZHANG Yunxiao
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:12:36
OmpA-HIV aspartyl protease
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K533003_sequence (Version 1) |
Description
OmpA is used to present HIV aspartyl protease outside the E. Coli membrane so that it can cut other proteins with the specific sequence outside in the media.Notes
Since HIV protease is a dimer, dimerization is a great concern to its function. Function of this protease is tested in BL21.Source
HIV protease sequence is from former part BBa_I712667.OmpA sequence is from BL21(DE3) e. Coli strain.
| Sequence Annotation | Location | Component / Role(s) |
| HIV protease START STOP BBa_I712667 T7 lacI OmpA rbs | 633,935 87,89 933,935 633,935 1,18 20,44 87,632 75,79 | CDS feature/protein start_codon feature/start feature/stop stop_codon feature/BioBrick engineered_region feature/promoter promoter feature/operator operator feature/protein CDS ribosome_entry_site feature/rbs |