BBa_K533006

BBa_K533006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K533006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: ZHANG Yunxiao

Date created: 2011-09-28 11:00:00

Date modified: 2015-05-08 01:12:36

Multi-Proline-mCherry

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K533006_sequence (Version 1)

Description

mCherry protein with a his tag for purification and a proline rich peptide for association with SH3 domain.

Notes

proline rich sequence is much longer than the length required for SH3 binding, for easier binding to SH3 domain.

Source

mCherry sequence is from Clontech plasmid pmCherry-N1 and multi-proline sequence is synthesized from a consensus sequence of SH3 binding sequence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
rbs	20,25	ribosome_entry_site, feature/rbs
T7	1,18	promoter, feature/promoter
mCherry	92,907	feature/protein, CDS
multi-proline	137,175	feature/tag, tag
His Tag	44,61	ribosome_entry_site, feature/rbs
stop	905,907	stop_codon, feature/stop

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K533006/1

Member of these Collections

• iGEM Parts Registry