BBa_K533009

BBa_K533009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K533009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: ZHANG Yunxiao

Date created: 2011-09-28 11:00:00

Date modified: 2015-05-08 01:12:36

Tar receptor weak expression cassette

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K533009_sequence (Version 1)

Description

Tar receptor is now under the drive of lacI promoter, a constitutive weak promoter. In addition, the Shine-Dalgarno sequence is removed to lower translation efficiency. Lower expression level ensures more specific chemotaxis and better engineering of movement control.

Notes

Lower the expression level is essential to chemotaxis.

Source

Kind gift from Dr. Goulian.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-35 (weak)	565,570	feature/promoter, promoter
lacI (part)	165,654	CDS, feature/cds
-10 (weak)	124,129	promoter, feature/promoter
-35	85,90	feature/promoter, promoter
-10 (weak)	585,590	promoter, feature/promoter
lacI inframe stop	660,662	feature/stop, stop_codon
lacI Initiation	165,167	start_codon, feature/start
Tar Initiation	655,657	feature/start, start_codon
lacI RBS	153,158	feature/rbs, ribosome_entry_site
Tar Receptor	655,2316	feature/protein, CDS
Tar Termination	2314,2316	stop_codon, feature/stop
bla	2867,3727	CDS, feature/cds

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K533009/1

Member of these Collections

• iGEM Parts Registry