Cpn10

BBa_K538000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K538000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bas Stringer (Sequence by Paul van Dieken)
Date created: 2011-07-27 11:00:00
Date modified: 2015-05-08 01:12:36

Cpn10 (O. antarctica)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K538000_sequence (Version 1)

Description

This part encodes the protein cochaperonin 10, which is part of the Cpn10/Cpn60 chaperonin system of Oleispira antarctica.

[More information will follow soon.]

Notes

The protein's sequence[1] was published by Ferrer et al. in 2004. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[2] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[3] was confirmed using the EMBOSS recoder[4], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.

1 - http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts
2 - http://openwetware.org/wiki/Biobrick_standard
3 - http://partsregistry.org/Help:Assembly_standard_10
4 - http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html

Source

De novo synthesis

Sequence Annotation Location Component / Role(s)
Start Codon
Double Stop Codon
1,3
292,297
feature/start start_codon
feature/stop stop_codon
igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K538000/1