BBa_K539714

BBa_K539714 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K539714
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yang, Yi-Pei
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:38

kivd (alpha-ketoisovalerate decarboxylase)



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K539714_sequence (Version 1)

Description

kivd is the coding sequence from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. This is part of the isobutanol biosynthesis pathway.

Notes

In order to design the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding region.However, there is one RE site, EcoR1, in kivd. The RE site in kivd has been modified by point mutation.

Source

It is cloned form Lactococcus lactis subsp. lactis KF147

Sequence Annotation Location Component / Role(s)
kivd coding sequence
1,1647
CDS feature/cds
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K539714/1