Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Melanie Geffroy, Philippe Thomas
Date created: 2011-09-14 11:00:00
Date modified: 2015-05-08 01:12:38
rcn-csgBAEFG, over-induces adherence in response to cobalt
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K540000_sequence (Version 1) |
Description
This parts associates the rcn promoter, whose expression increases with cobalt or nickel in the medium, with csgBAEFG, an operon that enables the production and secretion of curlis, which are adherent proteins.With this part, strains can become adherent in response to cobalt or nickel in the medium.
Notes
Several internal iGEM restriction sites were initially present in the genes : EcoR1 in csgEFG, Pst1 in csgBA and rcn. They were eliminated in such a way that it does not affect the expression of the genes.Source
Direct synthesis from Genecust, using the sequence of the genes as found in E.Coli MC4100 genome.| Sequence Annotation | Location | Component / Role(s) |
| rcnR -10rcnR -35rcnR rcnA perfect RBS1 Mut1 csgB csgA Mut2 perfect RBS2 csgE Mut3 csgF csgG | 8,280 395,400 422,427 401,435 442,454 169,175 457,911 952,1407 1322,1327 1415,1427 1431,1820 1812,1817 1845,2261 2288,3121 | feature/cds CDS feature/promoter promoter promoter feature/promoter feature/cds CDS feature/rbs ribosome_entry_site feature/mutation sequence_alteration CDS feature/cds CDS feature/cds feature/mutation sequence_alteration ribosome_entry_site feature/rbs feature/cds CDS sequence_alteration feature/mutation feature/cds CDS feature/cds CDS |