BBa_K541003

BBa_K541003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K541003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mustafa Elitok

Date created: 2011-08-15 11:00:00

Date modified: 2015-05-08 01:12:38

Reflectin 1A with promoter and RBS

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K541003_sequence (Version 1)

Description

Reflectin is a self-assembling protein that has the ability to reflect the sun light which depends on the thickness of the protein layer. Therefore, we thought that this ability could be used as a novel reporter for B.subtilis and E.coli.

Notes

Optimized for both E.coli and B.subtilis

Source

Cephalopod

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	feature/BioBrick, engineered_region
end of LacI coding region (inactive)	1,88	feature/cds, CDS
CAP binding site	89,126	feature/binding, non_covalent_binding_site
-35	137,142	promoter, feature/promoter
-10	161,166	feature/promoter, promoter
LacI binding site	166,200	feature/binding, non_covalent_binding_site
start	173,173	feature/start, start_codon
conserved	213,216	feature/conserved, conserved_region
BBa_B0034	209,220	engineered_region, feature/BioBrick
Reflectin	227,1081	CDS, feature/protein
BBa_K541013	227,1081	engineered_region, feature/BioBrick

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K541003/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• //chassis/prokaryote/ecoli
• iGEM Parts Registry