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BBa_K542003

BBa_K542003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K542003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anthony Vuong

Date created: 2011-09-22 11:00:00

Date modified: 2015-05-08 01:12:38

pBAD Regulated TetR

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Details

Types	DnaRegion
Roles	Inverter engineered_region
Sequences	BBa_K542003_sequence (Version 1)

Description

Made by assembling BBa_I13453 with BBa_P0440.

Production of TetR is regulated by the pBAD promoter(BBa_I13453). TetR is an inhibitor of the constitutively "on" promoter pTet--BBa_R0040). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose.

Arabinose -> pBAD "on" -> TetR -> pTet "off"

Intermediate part to assemble the "Co-localization Construct".

Notes

Intermediate part to assemble the "Co-localization Construct".

Source

BBa_I13453 and BBa_P0440 were both obtained from iGEM distribution plates.

Components

Access	Instance	Definition
public public	BBa_I13453 BBa_P0440	BBa_I13453 BBa_P0440

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I13453 BBa_P0440	1,130 139,978	BBa_I13453 BBa_P0440

Other Properties

igem#experience	Works
igem#partStatus	Released HQ 2013
igem#sampleStatus	In stock
igem#status	Available
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K542003/1