BBa_K542007

BBa_K542007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K542007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anthony Vuong

Date created: 2011-09-23 11:00:00

Date modified: 2015-05-08 01:12:39

Unregulated TetR with pTet Regulated Arg-tagged ECFP

Details

• Types: DnaRegion
• Roles: engineered_region; Intermediate
• Sequences: BBa_K542007_sequence (Version 1)

Description

Made by assembling BBa_P0440 with BBa_K331033.


The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP will be produced constitutively. By placing an inducible promoter upstream of this part, expression of the Arg-tagged ECFP will be regulated inversely. If the pLacI promoter (BBa_R0010) is placed upstream, the presence of IPTG will cease Arg-tagged ECFP expression.

Intermediate part to assemble the "Co-localization Construct".

Notes

Intermediate part to assemble the "Co-localization Construct".

Source

BBa_P0440 was obtained from iGEM Distribution plates and BBa_K331033 was assembled by the Lethbridge 2010 team.

Components

Access	Instance	Definition
public	BBa_P0440	BBa_P0440
public	BBa_K331033	BBa_K331033

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_P0440	1,840	BBa_P0440
BBa_K331033	849,1684	BBa_K331033

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K542007/1

Member of these Collections

• iGEM Parts Registry