Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anthony Vuong
Date created: 2011-09-23 11:00:00
Date modified: 2015-05-08 01:12:39
Unregulated TetR with pTet Regulated Arg-tagged ECFP
| Types | DnaRegion |
| Roles | engineered_region Intermediate |
| Sequences | BBa_K542007_sequence (Version 1) |
Description
Made by assembling BBa_P0440 with BBa_K331033.The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP will be produced constitutively. By placing an inducible promoter upstream of this part, expression of the Arg-tagged ECFP will be regulated inversely. If the pLacI promoter (BBa_R0010) is placed upstream, the presence of IPTG will cease Arg-tagged ECFP expression.
Intermediate part to assemble the "Co-localization Construct".
Notes
Intermediate part to assemble the "Co-localization Construct".Source
BBa_P0440 was obtained from iGEM Distribution plates and BBa_K331033 was assembled by the Lethbridge 2010 team.| Access | Instance | Definition |
| public public | BBa_P0440 BBa_K331033 | BBa_P0440 BBa_K331033 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_P0440 BBa_K331033 | 1,840 849,1684 | BBa_P0440 BBa_K331033 |