BBa_K544011

BBa_K544011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K544011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leung Man Hong
Date created: 2011-09-29 11:00:00
Date modified: 2015-05-08 01:12:39

tetR-HNS (2-90) fusion protein (Consitutive Promoter: BBa-J23103)



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K544011_sequence (Version 1)

Description

The tetR-HNS2-90 protein fuses the polymerization demain of histone-like nucleoid-structuring (H-NS) protein to tetR. Thus, in E.coli, when TetR-HNS2-90 binds the TetR-specific DNA motif, it will recruit native H-NS to bind to adjacent DNA, which may lead to local gene silencing. This fusion protein is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106). Experimental data indicates that TetR-HNS2-90 is more effective than TetR on transcription repression.

Notes

There is a internal Pst1 site at in H-NS coding region

Source

H-NS2-90 coding region was cloned from E. coli K-12 MG1655 genomic DNA.

Sequence Annotation Location Component / Role(s)
BBa_J23103
BBa_B0034
tetR-HNS(2-90)
1,35
44,55
62,949
promoter feature/promoter
feature/rbs ribosome_entry_site
feature/cds CDS
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K544011/1