BBa_K544013

BBa_K544013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K544013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Leung Man Hong

Date created: 2011-09-29 11:00:00

Date modified: 2015-05-08 01:12:39

tetR-HNS (2-90) fusion protein (Consitutive Promoter: BBa-J23109)

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K544013_sequence (Version 1)

Description

The tetR-HNS2-90 protein fuses the polymerization demain of histone-like nucleoid-structuring (H-NS) protein to tetR. Thus, in E.coli, when TetR-HNS2-90 binds the TetR-specific DNA motif, it will recruit native H-NS to bind to adjacent DNA, which may lead to local gene silencing. This fusion protein is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106). Experimental data indicates that TetR-HNS2-90 is more effective than TetR on transcription repression.

Notes

There is a internal Pst1 site at in H-NS coding region

Source

H-NS2-90 coding region was cloned from E. coli K-12 MG1655 genomic DNA.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23109	1,35	feature/promoter, promoter
BBa_B0034	44,55	feature/rbs, ribosome_entry_site
tetR-HNS(2-90)	62,949	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K544013/1

Member of these Collections

• iGEM Parts Registry