Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brendan Ryback, Matthijn Hesselman
Date created: 2011-09-15 11:00:00
Date modified: 2015-05-08 01:12:39
Constitutive (tetR repressible) LacI and RFP expression
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K546547_sequence (Version 1) |
Description
This part constitutively expresses LacI and RFP except when repressed by TetR. When cells containing this part are exposed to the inducer molecule IPTG, it can be used to regulate lac-repressible promoters. It was designed to function as an IPTG-controllable tuner for the Double Tunable Synchronized Oscillator. It is advisable to operate this part on a low or medium copy plasmid.Notes
No specific considerations.Source
Composite part of R0040 and J24675| Access | Instance | Definition |
| public public | BBa_R0040 BBa_J24675 | p(tetR) BBa_J24675 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 BBa_J24675 | 1,54 63,2110 | p(tetR) BBa_J24675 |