BBa_K549038

BBa_K549038 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K549038

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2011-09-20 11:00:00

Date modified: 2015-05-08 01:12:40

Promotor prcnA of rcnA with RcnR-binding site and 50nt rcnA

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K549038_sequence (Version 1)

Description

This is the promoter of rcnA, called prcnA. It has a binding site for the regulator RcnR, which originates in E. coli. In presence of Ni(II), Ni(II) binds to RcnR, RcnR binds to the promoter and activates transcription.
Co(II) can also be a stimulus, but a weaker one.
This promoter contains the first ca. 50 nt of rcnA, and therefore is used for transcriptional fusion with genes with RBS.

Notes

This promoter contains the first ca. 50 nt of rcnA, and therefore is used for transcriptional fusion with genes with RBS. This BB can be used for measurement of Ni(II)-concentration.

Source

It originates from E.coli K12 MG 1655 gDNA.

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K549038/1

Member of these Collections

• iGEM Parts Registry