pINDEL

BBa_K551001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K551001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: ULB-Brussels team

Date created: 2011-09-18 11:00:00

Date modified: 2015-05-08 01:12:40

plasmid of insertion and deletion

Details

• Types: DnaRegion
• Roles: Plasmid; plasmid
• Sequences: BBa_K551001_sequence (Version 1)

Description

Plasmid of insertion and deletion of genes

Notes

The thermosensitive origin of replication made it slow to grow

Source

It comes from pKD46 which was modified by adding the flipase function to it

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
AmpR	9818,10678	feature/cds, CDS
URA3	707,1510	CDS, feature/cds
AraC	2504,3392	CDS, feature/cds
gam	3737,4152	CDS, feature/cds
bet	4159,4944	feature/cds, CDS
exo	4941,5621	feature/cds, CDS
Flipase	5805,7076	feature/cds, CDS
CI857ts	7076,7890	CDS, feature/cds
RepA101ts	7936,8889	feature/cds, CDS
OriR101	8894,9634	sequence_feature, feature/misc
pARAb	3658,3685	promoter, feature/promoter

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K551001/1

Member of these Collections

• //function/recombination/flp
• iGEM Parts Registry