Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonathan Herrmann
Date created: 2011-09-23 11:00:00
Date modified: 2015-05-08 01:12:40
CrtE Gene-Codon Optimized for Yeast
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K552001_sequence (Version 1) |
Description
This DNA sequence codes for the enzyme CrtE, also known as geranylgeranyl diphosphate synthase. CrtE serves as the first step in carotenoid biosynthesis in Saccharomyces cerevisiae by converting farnesyl diphosphate (which is naturally found in yeast) to geranylgeranyl diphosphate.Notes
While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.Source
The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm| Sequence Annotation | Location | Component / Role(s) |
| PGK Promoter Start Codon CrtE Stop Codon ADH1 Terminator | 1,380 381,383 381,1511 1508,1510 1512,1639 | feature/promoter promoter start_codon feature/start CDS feature/cds stop_codon feature/stop sequence_feature feature/misc |