BBa_K558000

BBa_K558000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K558000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jovanna Casas

Date created: 2011-08-24 11:00:00

Date modified: 2015-05-08 01:12:41

PDC/ADH coding regions

Details

• Types: DnaRegion
• Roles: engineered_region; Intermediate
• Sequences: BBa_K558000_sequence (Version 1)

Description

This intermediate part (along with a promoter) was designed for ethanol production. The ethanol produced is to be used as an additive for biofuel.

The pyruvate decarboxylase gene converts pyruvate to acetaldehyde. Acetaldehyde is then converted to ethanol by alcohol dehydrogenase.

Notes

none

Source

The sequence for these genes is based on Zymomonas mobilis, a microorganism that naturally produces ethanol.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS	1,16	feature/rbs, ribosome_entry_site
PDC Coding Region	17,1720	feature/cds, CDS
2x Stop	1721,1725	feature/stop, stop_codon
Not I Cut Site	1728,1735	feature/misc, sequence_feature
Xho I Cut Site	1736,1741	sequence_feature, feature/misc
ADH Coding Region	1757,2905	feature/cds, CDS
4x Stop	2906,2917	feature/stop, stop_codon
BBa_K558000	2918,3013	feature/BioBrick, engineered_region
Stuffer Region	1727,1756	sequence_feature, feature/misc
start	17,19	start_codon, feature/start
start	1757,1759	feature/start, start_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K558000/1

Member of these Collections

• iGEM Parts Registry