Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhang Chao
Date created: 2011-10-25 11:00:00
Date modified: 2015-05-08 01:12:41
BsaI Golden Gate Assembly No.2
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K560013_sequence (Version 1) |
Description
The second vector of the Golden gate series (adapted from psb1c3)This vector expedites golden gate cloning with BBa_K560012, BBa_K560014 and BBa_K560015. To make this set of vector compatible with the standard biobricks, we have maintained PstI and XbaI recognition sites. Flanking these two sites are the cleavage and recognition sites of BsaI, a kind of Type IIS restriction endonuclease. This kind of restriction endonucleases cleaves outside their recognition sites thus creating different sticky ends. If you want to assemble four biobricks at a time, you can put the biobricks in order into corresponding vector by the digestion of PstI and Xbal. Then mix these assembled vectors and a backbone (PFUS4 from TALEN golden gate kit). Under the effect of ligase and Bsal, these biobricks will have sticky ends nose to tails and ligate in order in a single reaction.