Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2011-09-15 11:00:00
Date modified: 2015-05-08 01:12:41
Salty_PduN BMC Component
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K562005_sequence (Version 1) |
Description
This is a composite part comprising a constituive promoter (identical to that from the basic part BBa_K562000), which is the tatABCD promoter from E. coli K-12, driving production of the PduN protein from Salmonella enterica serovar Typhimurium LT2. This is a component of the Salmonella bacterial microcompartment (BMC). The PduN gene product carries a C-terminal 6-His affinity/epitope tag. Production of the PduN protein has been verified by Western immunoblotting (anti-penta-His) and production of PduN has been confirmed by 35S-Met labelling. The protein has also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-N in the Sargent Laboratory, Dundee, UK.Notes
The fragment carries an engineered BamHI site at its extreme 3' end.Source
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K562000 synthetic RBS pduN gene Hexa-Histidine Tag | 1,103 107,112 119,391 392,409 | engineered_region feature/BioBrick feature/rbs ribosome_entry_site CDS feature/cds feature/misc sequence_feature |