Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2011-09-17 11:00:00
Date modified: 2015-05-08 01:12:41
Salty_PduD40-Limonene
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K562015_sequence (Version 1) |
Description
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to limonene synthase from Citrus limon (from part BBa_I742111). The limonene synthase gene product also carries a C-terminal HA epitope tag. Production of PduD40-Lim has been verified by Western immunoblotting (anti-HA). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-Lim in the Sargent Laboratory, Dundee, UK.Notes
A HindIII site from the limonene synthase gene was removed by site-directed mutagenesis. This will allow future cloning into other compatible vectors.Source
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and biobrick BBa_I742111.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K562002 synthetic RBS PduD40 Tag BBa_I742111 limonene synthase HA epitope tag | 1,244 107,112 119,238 245,1906 245,1906 1907,1933 | feature/BioBrick engineered_region ribosome_entry_site feature/rbs CDS feature/cds engineered_region feature/BioBrick CDS feature/cds tag feature/tag |