Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2011-09-17 11:00:00
Date modified: 2015-05-08 01:12:41
Salty_PduD40-Asp
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K562017_sequence (Version 1) |
Description
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to L-aspartate ammonia-lyase (AspA) from Escherichia coli (from part BBa_C0083). The AspA gene product also carries a C-terminal HA epitope tag. Production of PduD20-mCherry has been verified by Western immunoblotting (anti-HA). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-Asp in the Sargent Laboratory, Dundee, UK.Notes
NoneSource
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and part BBa_C0083.| Sequence Annotation | Location | Component / Role(s) |
| HA epitope tag BBa_K562002 synthetic RBS PduD40 Tag AspA BBa_C0083 | 1754,1780 1,244 107,112 119,238 245,1753 245,1753 | feature/tag tag feature/BioBrick engineered_region ribosome_entry_site feature/rbs tag feature/tag feature/cds CDS feature/BioBrick engineered_region |