Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Boxuan Zeng
Date created: 2011-10-04 11:00:00
Date modified: 2015-05-08 01:12:42
Tor2 with L1415P mutation
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K563011_sequence (Version 1) |
Description
The Tor2 with L1415P mutation will be insensitive to amino starvation and inhibitor. Its activity is more stable than tor2 without mutation.Notes
According to the mutation Tor in mammalian cell, we found the mutation of Tor2 in S. cerevisiae. When we mutated BBa_K563010, we found that it is necessary to use more efficient DNA polymerase, to ensure the quality of the PCR product. DpnI is necessary to deal with the PCR product to degrade the plasmid which is not mutated. Anyone can take this part by cutting the plasmid with NcoI and PstI.Source
From the parts BBa_K563010, mutated by circular PCR.| Sequence Annotation | Location | Component / Role(s) |
| L1415P mutation | 4825,4827 | feature/s_mutation point_mutation |