BBa_K563013

BBa_K563013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K563013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Boxuan Zeng
Date created: 2011-10-04 11:00:00
Date modified: 2015-05-08 01:12:42

Tor2 with mutation I1957T and E2360K



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K563013_sequence (Version 1)

Description

It has the mutation of I1957T and E2360K. It has high kinase activity, resist to rapamycin, amino starvation and multi-inhibitor. It will phosphorylate downstream transcription factors,and inhibit their nuclear location. In our project when inhibitors exist, TORC1 is inactive, mutated Tor will help it grow in the environment.

Notes

Same to BBa_K563012

Source

We mutate BBa_K563010 by circular PCR.

Sequence Annotation Location Component / Role(s)
I1957T
E2360K
6451,6453
7660,7662
feature/mutation sequence_alteration
sequence_alteration feature/mutation
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K563013/1