BBa_K563055

BBa_K563055 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K563055

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Guo Jing

Date created: 2011-10-04 11:00:00

Date modified: 2015-05-08 01:12:42

pSB1A11, reconstructed from pSB1A2 with NcoI, XhoI, BamHI and PstI

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_K563055_sequence (Version 1)

Description

pSB1A2 is a existing BioBrick, and we reconstruct it to make our connection easier. We add NcoI, XhoI, BamHI, and PstI into the vector.

Notes

In order to make our mutation work easier, we separate Tor2 into three parts. so we add fout certain restriction sites into the vector to make the connection easier to conduct.

Source

pSB1A2 is from BioBrick

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
NcoI	121,126	feature/misc, sequence_feature
XhoI	133,138	sequence_feature, feature/misc
BamHI	153,158	sequence_feature, feature/misc
PstI	167,172	sequence_feature, feature/misc

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K563055/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors